› 1gqv

X-ray diffraction
0.98Å resolution

Atomic Resolution (0.98A) Structure of Eosinophil-Derived Neurotoxin

Released:
DOI: 10.2210/pdb1gqv/pdb
PDB entry 1gqv coloured by chain, front view.
PDB entry 1gqv coloured by chain, side view.
PDB entry 1gqv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Atomic resolution (0.98 A) structure of eosinophil-derived neurotoxin.
Swaminathan GJ, Holloway DE, Veluraja K, Acharya KR
Biochemistry 41 3341-52 (2002)
PMID: 11876642

Function and Biology Details

Reaction catalysed: 
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145194 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Non-secretory ribonuclease Chain: A
Molecule details ›
Chain: A
Length: 135 amino acids
Theoretical weight: 15.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P10153 (Residues: 28-161; Coverage: 100%)
Gene names: EDN, RNASE2, RNS2
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

1 bound ligand:
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P212121
Unit cell:
a: 53.024Å b: 57.477Å c: 42.398Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.116 not available 0.144
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Eosinophil-derived neurotoxin / RNase 2: connecting the past, the present and the future.
Rosenberg HF. (2008)
4 more

25 mentions without citation

Antimicrobial Peptides and Proteins: From Nature's Reservoir to the Laboratory and Beyond.
Sarkar et al. (2021)
24 more