PDBe 1gns

X-ray diffraction
1.8Å resolution

SUBTILISIN BPN'

Released:

Function and Biology Details

Reaction catalysed:
Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Subtilisin BPN' Chain: A
Molecule details ›
Chain: A
Length: 263 amino acids
Theoretical weight: 26.4 KDa
Source organism: Bacillus amyloliquefaciens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00782 (Residues: 111-382; Coverage: 75%)
Gene name: apr
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 41.17Å b: 78.06Å c: 36.7Å
α: 90° β: 114.6° γ: 90°
R-values:
R R work R free
0.165 0.165 not available
Expression system: Escherichia coli BL21(DE3)