PDBe 1gkm

X-ray diffraction
1Å resolution

HISTIDINE AMMONIA-LYASE (HAL) FROM PSEUDOMONAS PUTIDA INHIBITED WITH L-CYSTEINE

Released:

Function and Biology Details

Reaction catalysed:
L-histidine = urocanate + NH(3). 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Histidine ammonia-lyase Chain: A
Molecule details ›
Chain: A
Length: 507 amino acids
Theoretical weight: 53.62 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P21310 (Residues: 2-510; Coverage: 100%)
Gene name: hutH
Sequence domains: Aromatic amino acid lyase
Structure domains:

Ligands and Environments


1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: I222
Unit cell:
a: 79.267Å b: 116.788Å c: 129.532Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.119 not available 0.135
Expression system: Escherichia coli BL21(DE3)