Function and Biology

CRYSTAL STRUCTURE OF ADENYLOSUCCINATE SYNTHETASE FROM ESCHERICHIA COLI COMPLEXED WITH GDP, IMP, HADACIDIN, NO3-, AND MG2+. DATA COLLECTED AT 100K (PH 6.5)

Source organism: Escherichia coli
Biochemical function: nucleotide binding
Biological process: purine nucleotide biosynthetic process
Cellular component: membrane

EC 6.3.4.4: Adenylosuccinate synthase

Reaction catalysed:
GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)- AMP.
Systematic name:
IMP:L-aspartate ligase (GDP-forming)
Alternative Name(s):
  • IMP--aspartate ligase
  • Adenylosuccinate synthetase
  • Succino-AMP synthetase
  • Succinoadenylic kinosynthetase

Sequence family

CATH Protein family (Pfam)
PF00709
Domain description: Adenylosuccinate synthetase
Occurring in:
  1. Adenylosuccinate synthetase
1 copy of Pfam domain PF00709 (Adenylosuccinate synthetase) in Adenylosuccinate synthetase in PDB 1gim.

CATH InterPro annotations
IPR001114
Domain description: Adenylosuccinate synthetase
Occurring in:
  1. Adenylosuccinate synthetase
IPR018220
Domain description: Adenylosuccinate synthase, GTP-binding site
Occurring in:
  1. Adenylosuccinate synthetase
IPR033128
Domain description: Adenylosuccinate synthase, active site
Occurring in:
  1. Adenylosuccinate synthetase
IPR027417
Domain description: P-loop containing nucleoside triphosphate hydrolase
Occurring in:
  1. Adenylosuccinate synthetase

Structure domains

CATH CATH domains
3.40.440.10
Class: Alpha Beta
Architecture: 3-Layer(aba) Sandwich
Topology: Adenylosuccinate Synthetase; Chain A, domain 1
Homology: Adenylosuccinate Synthetase, subunit A, domain 1
Occurring in:
  1. Adenylosuccinate synthetase
1 copy of CATH domain 3.40.440.10 (Adenylosuccinate Synthetase; Chain A, domain 1) in Adenylosuccinate synthetase in PDB 1gim.
1.10.300.10
Class: Mainly Alpha
Architecture: Orthogonal Bundle
Topology: Adenylosuccinate Synthetase, subunit A; domain 2
Homology: Adenylosuccinate Synthetase, subunit A, domain 2
Occurring in:
  1. Adenylosuccinate synthetase
1 copy of CATH domain 1.10.300.10 (Adenylosuccinate Synthetase, subunit A; domain 2) in Adenylosuccinate synthetase in PDB 1gim.
3.90.170.10
Class: Alpha Beta
Architecture: Alpha-Beta Complex
Topology: Adenylosuccinate Synthetase; Chain A, domain 3
Homology: Adenylosuccinate Synthetase, subunit A, domain 3
Occurring in:
  1. Adenylosuccinate synthetase
1 copy of CATH domain 3.90.170.10 (Adenylosuccinate Synthetase; Chain A, domain 3) in Adenylosuccinate synthetase in PDB 1gim.
SCOP SCOP annotation
52652
Class: Alpha and beta proteins (a/b)
Fold: P-loop containing nucleoside triphosphate hydrolases
Superfamily: P-loop containing nucleoside triphosphate hydrolases
Occurring in:
  1. Adenylosuccinate synthetase
1 copy of SCOP domain 52652 (Nitrogenase iron protein-like) in Adenylosuccinate synthetase in PDB 1gim.