Function and Biology

GLYCYL ENDOPEPTIDASE-COMPLEX WITH BENZYLOXYCARBONYL-LEUCINE-VALINE-GLYCINE-METHYLENE COVALENTLY BOUND TO CYSTEINE 25

Source organisms:
Biochemical function: cysteine-type peptidase activity
Biological process: proteolysis
Cellular component: not assigned

EC 3.4.22.25: Glycyl endopeptidase

Reaction catalysed:
Preferential cleavage: Gly-|-Xaa, in proteins and in small molecule substrates.
Comments:
  • From the papaya plant, Carica papaya.
  • Not inhibited by chicken cystatin, unlike most other homologs of papain.
  • Belongs to peptidase family C1.
Alternative Name(s):
  • Papaya proteinase 4
  • PPIV
  • Chymopapain
  • Chymopapain M
  • Glycine-specific proteinase
  • Papaya peptidase B
  • Papaya proteinase IV

GO terms

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Sequence family

Pfam Protein family (Pfam)
PF00112
Domain description: Papain family cysteine protease
Occurring in:
  1. Papaya proteinase 4
1 copy of Pfam domain PF00112 (Papain family cysteine protease) in Papaya proteinase 4 in PDB 1gec.

InterPro InterPro annotations
IPR000668
Domain description: Peptidase C1A, papain C-terminal
Occurring in:
  1. Papaya proteinase 4
IPR025660
Domain description: Cysteine peptidase, histidine active site
Occurring in:
  1. Papaya proteinase 4
IPR025661
Domain description: Cysteine peptidase, asparagine active site
Occurring in:
  1. Papaya proteinase 4
IPR000169
Domain description: Cysteine peptidase, cysteine active site
Occurring in:
  1. Papaya proteinase 4
IPR013128
Domain description: Peptidase C1A
Occurring in:
  1. Papaya proteinase 4

Structure domain

CATH CATH domain
3.90.70.10
Class: Alpha Beta
Architecture: Alpha-Beta Complex
Topology: Cathepsin B; Chain A
Homology: Cysteine proteinases
Occurring in:
  1. Papaya proteinase 4
1 copy of CATH domain 3.90.70.10 (Cathepsin B; Chain A) in Papaya proteinase 4 in PDB 1gec.
SCOP SCOP annotation
54002
Class: Alpha and beta proteins (a+b)
Fold: Cysteine proteinases
Superfamily: Cysteine proteinases
Occurring in:
  1. Papaya proteinase 4
1 copy of SCOP domain 54002 (Papain-like) in Papaya proteinase 4 in PDB 1gec.