› 1ge8

X-ray diffraction
2.1Å resolution

PROLIFERATING CELL NUCLEAR ANTIGEN (PCNA) HOMOLOG FROM PYROCOCCUS FURIOSUS

Released:
DOI: 10.2210/pdb1ge8/pdb
PDB entry 1ge8 coloured by chain, front view.
PDB entry 1ge8 coloured by chain, side view.
PDB entry 1ge8 coloured by chain, top view.
Source organism: Pyrococcus furiosus
Primary publication:
Crystal structure of an archaeal DNA sliding clamp: proliferating cell nuclear antigen from Pyrococcus furiosus.
Matsumiya S, Ishino Y, Morikawa K
Protein Sci 10 17-23 (2001)
PMID: 11266590

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130735 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA polymerase sliding clamp Chain: A
Molecule details ›
Chain: A
Length: 249 amino acids
Theoretical weight: 28.02 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O73947 (Residues: 1-249; Coverage: 100%)
Gene names: PF0983, pcn
Sequence domains:
Structure domains: Proliferating Cell Nuclear Antigen

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P63
Unit cell:
a: 89.682Å b: 89.682Å c: 63.269Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.228 0.225 0.253
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

20 review citations

Proliferating cell nuclear antigen (PCNA): a key factor in DNA replication and cell cycle regulation.
Strzalka et al. (2011)
19 more

17 mentions without citation

Replication clamps and clamp loaders.
Hedglin et al. (2013)
16 more