Function and Biology Details
Biochemical function:
Biological process:
Cellular component:
- not assigned
Sequence domains:
Structure domain:
Structure analysis Details
Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113863 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
2 distinct DNA molecules
Macromolecules (3 distinct):
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
Refinement method:
SIMULATED ANNEALING PROTOCOL IN X-PLOR 3.1 WAS CARRIED OUT TO OBTAIN 25 STRUCTURES. MINIMIZED MEAN STRUCTURE OF THEM WAS FURTHER REVISED BY A 300 PS RESTRAINED MOLECULAR DYNAMICAL CALCULATION IN THE PRESENCE OF APPROXIMATELY 5000 TIP3P WATER MOLSCULES, USING CORNELL FORCE FIELD IN AMBER 4.1. THE MEAN CO-ORDINATE OF 200 STRUCTURES OBTAINED AFTER THE EQUILIBRIUM (100 PS) WAS ENERGY-MINIMIZED.
Expression systems:
- Escherichia coli BL21(DE3)
- Not provided