PDBe 1gcb

X-ray diffraction
2.2Å resolution

GAL6, YEAST BLEOMYCIN HYDROLASE DNA-BINDING PROTEASE (THIOL)

Released:

Function and Biology Details

Reaction catalysed:
Inactivates bleomycin B2 (a cytotoxic glycometallopeptide) by hydrolysis of a carboxyamide bond of beta-aminoalanine, but also shows general aminopeptidase activity. The specificity varies somewhat with source, but amino acid arylamides of Met, Leu and Ala are preferred. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine proteinase 1, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 454 amino acids
Theoretical weight: 52.15 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q01532 (Residues: 30-483; Coverage: 94%)
Gene names: BLH1, GAL6, LAP3, N1118, YCP1, YNL239W
Sequence domains: Peptidase C1-like family
Structure domains: Cysteine proteinases

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P6322
Unit cell:
a: 151.15Å b: 151.15Å c: 89.88Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.209 0.209 0.274
Expression system: Saccharomyces cerevisiae