PDBe 1gag

X-ray diffraction
2.7Å resolution

CRYSTAL STRUCTURE OF THE INSULIN RECEPTOR KINASE IN COMPLEX WITH A BISUBSTRATE INHIBITOR

Released:
Primary publication:
Mechanism-based design of a protein kinase inhibitor.
Nat. Struct. Biol. 8 37-41 (2001)
PMID: 11135668

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
BISUBSTRATE PEPTIDE INHIBITOR Chain: B
Molecule details ›
Chain: B
Length: 13 amino acids
Theoretical weight: 1.32 KDa
Source organism: Synthetic construct
Expression system: Not provided
Insulin receptor subunit beta Chain: A
Molecule details ›
Chain: A
Length: 306 amino acids
Theoretical weight: 35.03 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P06213 (Residues: 1005-1310; Coverage: 23%)
Gene name: INSR
Sequence domains: Protein tyrosine kinase
Structure domains:

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P3221
Unit cell:
a: 66.3Å b: 66.3Å c: 138.1Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.214 0.214 0.269
Expression systems:
  • Not provided
  • Spodoptera frugiperda