PDBe 1ga5

X-ray diffraction
2.4Å resolution

CRYSTAL STRUCTURE OF THE ORPHAN NUCLEAR RECEPTOR REV-ERB(ALPHA) DNA-BINDING DOMAIN BOUND TO ITS COGNATE RESPONSE ELEMENT

Released:
Source organism: Homo sapiens
Primary publication:
DNA deformability as a recognition feature in the reverb response element.
Biochemistry 40 12833-43 (2001)
PMID: 11669620

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Nuclear receptor subfamily 1 group D member 1 Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 94 amino acids
Theoretical weight: 10.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P20393 (Residues: 123-146, 148-216; Coverage: 15%)
Gene names: EAR1, HREV, NR1D1, THRAL
Sequence domains: Zinc finger, C4 type (two domains)
Structure domains: Erythroid Transcription Factor GATA-1, subunit A
5'-D(*CP*AP*AP*CP*TP*AP*GP*GP*TP*CP*AP*CP*TP*AP*GP*GP*TP*CP*AP*G)-3' Chains: C, G
Molecule details ›
Chains: C, G
Length: 20 nucleotides
Theoretical weight: 6.14 KDa
5'-D(*CP*TP*GP*AP*CP*CP*TP*AP*GP*TP*GP*AP*CP*CP*TP*AP*GP*TP*(5IT)P*G)-3' Chains: D, H
Molecule details ›
Chains: D, H
Length: 20 nucleotides
Theoretical weight: 6.24 KDa

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P1
Unit cell:
a: 44.92Å b: 52.02Å c: 78.88Å
α: 85.84° β: 76.61° γ: 74.48°
R-values:
R R work R free
0.236 0.253 0.299
Expression system: Escherichia coli BL21(DE3)