PDBe 1g99

X-ray diffraction
2.5Å resolution

AN ANCIENT ENZYME: ACETATE KINASE FROM METHANOSARCINA THERMOPHILA

Released:

Function and Biology Details

Reaction catalysed:
ATP + acetate = ADP + acetyl phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetate kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 408 amino acids
Theoretical weight: 44.39 KDa
Source organism: Methanosarcina thermophila
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P38502 (Residues: 1-408; Coverage: 100%)
Gene names: ack, ackA
Sequence domains: Acetokinase family
Structure domains: Nucleotidyltransferase; domain 5

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2
Unit cell:
a: 181.3Å b: 67.4Å c: 82.6Å
α: 90° β: 102.9° γ: 90°
R-values:
R R work R free
0.147 0.147 0.188
Expression system: Escherichia coli BL21(DE3)