› 1g7k

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF DSRED, A RED FLUORESCENT PROTEIN FROM DISCOSOMA SP. RED

Released:
DOI: 10.2210/pdb1g7k/pdb
PDB entry 1g7k coloured by chain, front view.
PDB entry 1g7k coloured by chain, side view.
PDB entry 1g7k coloured by chain, top view.
Source organism: Discosoma sp.
Primary publication:
Refined crystal structure of DsRed, a red fluorescent protein from coral, at 2.0-A resolution.
Yarbrough D, Wachter RM, Kallio K, Matz MV, Remington SJ
Proc Natl Acad Sci U S A 98 462-7 (2001)
PMID: 11209050

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193624 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Red fluorescent protein drFP583 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 234 amino acids
Theoretical weight: 27.55 KDa
Source organism: Discosoma sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9U6Y8 (Residues: 2-225; Coverage: 100%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
2 modified residues:
Ligand MSE 28 x MSE
Ligand CRQ 4 x CRQ

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21
Unit cell:
a: 56.366Å b: 112.27Å c: 70.691Å
α: 90° β: 93.81° γ: 90°
R-values:
R R work R free
0.166 0.162 0.249
Expression system: Escherichia coli

Quick links

Citations

34 review citations

Protein posttranslational modifications: the chemistry of proteome diversifications.
Walsh et al. (2005)
33 more

29 mentions without citation

The fluorescent protein palette: tools for cellular imaging.
Day et al. (2009)
28 more