› 1g5a

X-ray diffraction
1.4Å resolution

AMYLOSUCRASE FROM NEISSERIA POLYSACCHAREA

Released:
DOI: 10.2210/pdb1g5a/pdb
PDB entry 1g5a coloured by chain, front view.
PDB entry 1g5a coloured by chain, side view.
PDB entry 1g5a coloured by chain, top view.
Source organism: Neisseria polysaccharea
Primary publication:
Amylosucrase, a glucan-synthesizing enzyme from the alpha-amylase family.
Skov LK, Mirza O, Henriksen A, De Montalk GP, Remaud-Simeon M, Sarçabal P, Willemot RM, Monsan P, Gajhede M
J Biol Chem 276 25273-8 (2001)
PMID: 11306569

Function and Biology Details

Reaction catalysed: 
Sucrose + ((1->4)-alpha-D-glucosyl)(n) = D-fructose + ((1->4)-alpha-D-glucosyl)(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195625 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amylosucrase Chain: A
Molecule details ›
Chain: A
Length: 628 amino acids
Theoretical weight: 71.56 KDa
Source organism: Neisseria polysaccharea
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9ZEU2 (Residues: 9-636; Coverage: 99%)
Gene name: ams
Sequence domains: Alpha amylase, catalytic domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand NA 1 x NA
Ligand TRS 1 x TRS
Ligand EPE 1 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14, ESRF BEAMLINE ID14-1
Spacegroup: P21212
Unit cell:
a: 95.765Å b: 117.11Å c: 60.965Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.189 0.204
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Structure-function relationships of glucansucrase and fructansucrase enzymes from lactic acid bacteria.
van Hijum et al. (2006)
8 more

12 mentions without citation

Versatile biotechnological applications of amylosucrase, a novel glucosyltransferase.
Seo et al. (2020)
11 more