PDBe 1g4y

X-ray diffraction
1.6Å resolution

1.60 A CRYSTAL STRUCTURE OF THE GATING DOMAIN FROM SMALL CONDUCTANCE POTASSIUM CHANNEL COMPLEXED WITH CALCIUM-CALMODULIN

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calmodulin-3 Chain: R
Molecule details ›
Chain: R
Length: 148 amino acids
Theoretical weight: 16.72 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0DP31 (Residues: 2-149; Coverage: 99%)
Gene names: CaMIII, Calm3, Cam3, Camc
Structure domains: EF-hand
Small conductance calcium-activated potassium channel protein 2 Chain: B
Molecule details ›
Chain: B
Length: 101 amino acids
Theoretical weight: 12.11 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P70604 (Residues: 396-487; Coverage: 16%)
Gene name: Kcnn2
Sequence domains: Calmodulin binding domain
Structure domains: Helix Hairpins

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: C2
Unit cell:
a: 77.65Å b: 66.69Å c: 64.69Å
α: 90° β: 93.7° γ: 90°
R-values:
R R work R free
0.212 0.221 0.251
Expression system: Escherichia coli BL21(DE3)