› 1g43

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE OF A FAMILY IIIA CBD FROM CLOSTRIDIUM CELLULOLYTICUM

Released:
DOI: 10.2210/pdb1g43/pdb
PDB entry 1g43 coloured by chain, front view.
PDB entry 1g43 coloured by chain, side view.
PDB entry 1g43 coloured by chain, top view.
Source organism: Ruminiclostridium cellulolyticum
Primary publication:
Structure of a family IIIa scaffoldin CBD from the cellulosome of Clostridium cellulolyticum at 2.2 A resolution.
Shimon LJ, Pagès S, Belaich A, Belaich JP, Bayer EA, Lamed R, Shoham Y, Frolow F
Acta Crystallogr D Biol Crystallogr 56 1560-8 (2000)
PMID: 11092922

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-175087 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CBM3 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 160 amino acids
Theoretical weight: 17.16 KDa
Source organism: Ruminiclostridium cellulolyticum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q45996 (Residues: 28-187; Coverage: 11%)
Gene name: cipC
Sequence domains: Cellulose binding domain
Structure domains: Endoglucanase-like

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-C
Spacegroup: P6522
Unit cell:
a: 46.86Å b: 46.86Å c: 244.121Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.19 0.264
Expression system: Escherichia coli

Quick links

Citations

4 review citations

The cellulosomes: multienzyme machines for degradation of plant cell wall polysaccharides.
Bayer et al. (2004)
3 more

6 mentions without citation

Carbohydrate-binding modules: fine-tuning polysaccharide recognition.
Boraston et al. (2004)
5 more