PDBe 1g2d

X-ray diffraction
2.2Å resolution

STRUCTURE OF A CYS2HIS2 ZINC FINGER/TATA BOX COMPLEX (CLONE #2)

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Early growth response protein 1 Chains: C, F
Molecule details ›
Chains: C, F
Length: 90 amino acids
Theoretical weight: 10.71 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08046 (Residues: 333-421; Coverage: 17%)
Gene names: Egr-1, Egr1, Krox-24
Sequence domains: Zinc finger, C2H2 type
Structure domains: Classic Zinc Finger
5'-D(*GP*AP*CP*GP*CP*TP*AP*TP*AP*AP*AP*AP*GP*GP*AP*G)-3' Chains: A, D
Molecule details ›
Chains: A, D
Length: 16 nucleotides
Theoretical weight: 4.98 KDa
Source organism: Synthetic construct
Expression system: Not provided
5'-D(*TP*CP*CP*TP*TP*TP*TP*AP*TP*AP*GP*CP*GP*TP*CP*C)-3' Chains: B, E
Molecule details ›
Chains: B, E
Length: 16 nucleotides
Theoretical weight: 4.82 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P64
Unit cell:
a: 105.1Å b: 105.1Å c: 104.6Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.225 0.225 0.262
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided