› 1fzy

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF SACCHAROMYCES CEREVISIAE UBIQUITIN CONJUGATING ENZYME 1

Released:
DOI: 10.2210/pdb1fzy/pdb
PDB entry 1fzy coloured by chain, front view.
PDB entry 1fzy coloured by chain, side view.
PDB entry 1fzy coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure of a conjugating enzyme-ubiquitin thiolester intermediate reveals a novel role for the ubiquitin tail.
Hamilton KS, Ellison MJ, Barber KR, Williams RS, Huzil JT, McKenna S, Ptak C, Glover M, Shaw GS
Structure 9 897-904 (2001)
PMID: 11591345

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149410 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin-conjugating enzyme E2 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 149 amino acids
Theoretical weight: 16.7 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P21734 (Residues: 2-150; Coverage: 69%)
Gene names: UBC1, YD9395.10, YDR177W
Sequence domains: Ubiquitin-conjugating enzyme
Structure domains: Ubiquitin Conjugating Enzyme

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 42.166Å b: 47.471Å c: 75.776Å
α: 90° β: 92.62° γ: 90°
R-values:
R R work R free
0.209 0.209 0.241
Expression system: Escherichia coli

Quick links

Citations

27 review citations

Ubiquitin: structures, functions, mechanisms.
Pickart et al. (2004)
26 more

5 mentions without citation

Systematic comparison of crystal and NMR protein structures deposited in the protein data bank.
Sikic et al. (2010)
4 more