› 1fu4

X-ray diffraction
2.36Å resolution

STRUCTURES OF GLYCOGEN PHOSPHORYLASE-INHIBITOR COMPLEXES AND THE IMPLICATIONS FOR STRUCTURE-BASED DRUG DESIGN

Released:
DOI: 10.2210/pdb1fu4/pdb
PDB entry 1fu4 coloured by chain, front view.
PDB entry 1fu4 coloured by chain, side view.
PDB entry 1fu4 coloured by chain, top view.
Source organism: Oryctolagus cuniculus
Primary publication:
Kinetic and crystallographic studies of glucopyranose spirohydantoin and glucopyranosylamine analogs inhibitors of glycogen phosphorylase.
Watson KA, Chrysina ED, Tsitsanou KE, Zographos SE, Archontis G, Fleet GW, Oikonomakos NG
Proteins 61 966-83 (2005)
PMID: 16222658

Function and Biology Details

Reaction catalysed: 
((1->4)-alpha-D-glucosyl)(n) + phosphate = ((1->4)-alpha-D-glucosyl)(n-1) + alpha-D-glucose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132556 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycogen phosphorylase, muscle form Chain: A
Molecule details ›
Chain: A
Length: 842 amino acids
Theoretical weight: 97.29 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P00489 (Residues: 2-843; Coverage: 100%)
Gene name: PYGM
Sequence domains: Carbohydrate phosphorylase
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments


Cofactor: Ligand PLP 1 x PLP
1 bound ligand:
Ligand GL9 1 x GL9
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: P43212
Unit cell:
a: 128.5Å b: 128.5Å c: 116.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.19 0.224

Quick links

Citations

2 review citations

Structural glycobiology: a game of snakes and ladders.
DeMarco et al. (2008)
1 more