PDBe 1fs1

X-ray diffraction
1.8Å resolution

INSIGHTS INTO SCF UBIQUITIN LIGASES FROM THE STRUCTURE OF THE SKP1-SKP2 COMPLEX

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
S-phase kinase-associated protein 2 Chains: A, C
Molecule details ›
Chains: A, C
Length: 53 amino acids
Theoretical weight: 6.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13309 (Residues: 89-141; Coverage: 13%)
Gene names: FBXL1, SKP2
Structure domains: Monooxygenase
S-phase kinase-associated protein 1 Chains: B, D
Molecule details ›
Chains: B, D
Length: 141 amino acids
Theoretical weight: 16.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P63208 (Residues: 1-147; Coverage: 87%)
Gene names: EMC19, OCP2, SKP1, SKP1A, TCEB1L
Structure domains: Potassium Channel Kv1.1; Chain A

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9A
Spacegroup: P21
Unit cell:
a: 46.54Å b: 41.6Å c: 87.23Å
α: 90° β: 93.42° γ: 90°
R-values:
R R work R free
0.218 0.218 0.274
Expression system: Escherichia coli