PDBe 1fqv

X-ray diffraction
2.8Å resolution

Insights into scf ubiquitin ligases from the structure of the skp1-skp2 complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
S-phase kinase-associated protein 1 Chains: B, D, F, H, J, L, N, P
Molecule details ›
Chains: B, D, F, H, J, L, N, P
Length: 149 amino acids
Theoretical weight: 16.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P63208 (Residues: 1-163; Coverage: 91%)
Gene names: EMC19, OCP2, SKP1, SKP1A, TCEB1L
Sequence domains: Skp1 family, dimerisation domain
Structure domains: Potassium Channel Kv1.1; Chain A
S-phase kinase-associated protein 2 Chains: A, C, E, G, I, K, M, O
Molecule details ›
Chains: A, C, E, G, I, K, M, O
Length: 336 amino acids
Theoretical weight: 37.92 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13309 (Residues: 89-424; Coverage: 79%)
Gene names: FBXL1, SKP2
Sequence domains: F-box-like
Structure domains: Ribonuclease Inhibitor

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: C2
Unit cell:
a: 262.7Å b: 148.2Å c: 133.3Å
α: 90° β: 120.03° γ: 90°
R-values:
R R work R free
0.275 0.275 0.314
Expression system: Escherichia coli