PDBe 1fos

X-ray diffraction
3.05Å resolution

TWO HUMAN C-FOS:C-JUN:DNA COMPLEXES

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Transcription factor AP-1 Chains: F, H
Molecule details ›
Chains: F, H
Length: 62 amino acids
Theoretical weight: 7.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P05412 (Residues: 254-315; Coverage: 19%)
Gene name: JUN
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Proto-oncogene c-Fos Chains: E, G
Molecule details ›
Chains: E, G
Length: 62 amino acids
Theoretical weight: 7.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01100 (Residues: 139-200; Coverage: 16%)
Gene names: FOS, G0S7
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
DNA (5'-D(*AP*AP*TP*GP*GP*AP*TP*GP*AP*GP*TP*CP*AP*TP*AP*GP*GP*AP*GP*A)-3') Chains: A, C
Molecule details ›
Chains: A, C
Length: 20 nucleotides
Theoretical weight: 6.27 KDa
DNA (5'-D(*TP*TP*CP*TP*CP*CP*TP*AP*TP*GP*AP*CP*TP*CP*AP*TP*CP*CP*AP*T)-3') Chains: B, D
Molecule details ›
Chains: B, D
Length: 20 nucleotides
Theoretical weight: 5.99 KDa

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21212
Unit cell:
a: 241.1Å b: 48.69Å c: 66Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.23 0.321
Expression system: Escherichia coli