PDBe 1fok

X-ray diffraction
2.8Å resolution

STRUCTURE OF RESTRICTION ENDONUCLEASE FOKI BOUND TO DNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Type-2 restriction enzyme FokI Chain: A
DNA (5'-D(*AP*TP*GP*AP*CP*TP*AP*GP*CP*GP*TP*TP*AP*TP*CP*AP*T P*CP*CP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 20 nucleotides
Theoretical weight: 6.11 KDa
Source organism: Unidentified
Expression system: Not provided
DNA (5'-D(*TP*CP*GP*GP*AP*TP*GP*AP*TP*AP*AP*CP*GP*CP*TP*AP*G P*TP*CP*A)-3') Chain: B
Molecule details ›
Chain: B
Length: 20 nucleotides
Theoretical weight: 6.16 KDa
Source organism: Planomicrobium okeanokoites
Expression system: Escherichia coli

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P21
Unit cell:
a: 65.59Å b: 119.34Å c: 71.52Å
α: 90° β: 101.42° γ: 90°
R-values:
R R work R free
0.214 0.214 0.296
Expression systems:
  • Not provided
  • Escherichia coli