PDBe 1foa

X-ray diffraction
1.8Å resolution

CRYSTAL STRUCTURE OF N-ACETYLGLUCOSAMINYLTRANSFERASE I

Released:

Function and Biology Details

Reaction catalysed:
UDP-N-acetyl-D-glucosamine + 3-(alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 3-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D- mannosyl-R. 
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase Chain: A
Molecule details ›
Chain: A
Length: 348 amino acids
Theoretical weight: 40.45 KDa
Source organism: Oryctolagus cuniculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P27115 (Residues: 106-447; Coverage: 77%)
Gene names: GNT1, MGAT1
Structure domains:

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: P212121
Unit cell:
a: 40.541Å b: 82.19Å c: 101.956Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.185 0.229
Expression system: Spodoptera frugiperda