PDBe 1fdy

X-ray diffraction
2.45Å resolution

N-ACETYLNEURAMINATE LYASE IN COMPLEX WITH HYDROXYPYRUVATE

Released:

Function and Biology Details

Reaction catalysed:
N-acetylneuraminate = N-acetyl-D-mannosamine + pyruvate. 
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acetylneuraminate lyase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 297 amino acids
Theoretical weight: 32.63 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6L4 (Residues: 1-297; Coverage: 100%)
Gene names: JW3194, b3225, nanA, npl
Sequence domains: Dihydrodipicolinate synthetase family
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MACSCIENCE M18XHF
Spacegroup: P3221
Unit cell:
a: 121Å b: 121Å c: 196.82Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.219 0.219 not available
Expression system: Escherichia coli