› 1f8w

X-ray diffraction
2.45Å resolution

CRYSTAL STRUCTURE OF NADH PEROXIDASE MUTANT: R303M

Released:
DOI: 10.2210/pdb1f8w/pdb
PDB entry 1f8w coloured by chain, front view.
PDB entry 1f8w coloured by chain, side view.
PDB entry 1f8w coloured by chain, top view.
Source organism: Enterococcus faecalis
Primary publication:
Analysis of the kinetic and redox properties of the NADH peroxidase R303M mutant: correlation with the crystal structure.
Crane EJ, Yeh JI, Luba J, Claiborne A
Biochemistry 39 10353-64 (2000)
PMID: 10956025

Function and Biology Details

Reaction catalysed: 
NADH + H(2)O(2) = NAD(+) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153338 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADH peroxidase Chain: A
Molecule details ›
Chain: A
Length: 447 amino acids
Theoretical weight: 49.59 KDa
Source organism: Enterococcus faecalis
Expression system: Escherichia coli
UniProt:
  • Canonical: P37062 (Residues: 1-447; Coverage: 100%)
Gene names: EF_1211, npr
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
No bound ligands
1 modified residue:
Ligand CSX 1 x CSX

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: I4122
Unit cell:
a: 155.18Å b: 155.18Å c: 189.41Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.281 0.196 0.234
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The physiological role of reversible methionine oxidation.
Drazic et al. (2014)
2 other articles

1 mention without citation

Crystallization and preliminary analysis of a water-forming NADH oxidase from Lactobacillus sanfranciscensis.
Lountos et al. (2004)