Function and Biology

HOLO-(ACYL CARRIER PROTEIN) SYNTHASE IN COMPLEX WITH COENZYME A AT 1.5A

Source organism: Bacillus subtilis
Biochemical function: transferase activity
Biological process: fatty acid biosynthetic process
Cellular component: cytoplasm

EC 2.7.8.7: Holo-[acyl-carrier-protein] synthase

Reaction catalysed:
CoA-(4'-phosphopantetheine) + apo-[acyl-carrier-protein] = adenosine 3',5'-bisphosphate + holo-[acyl-carrier-protein].
Comments:
  • All polyketide synthases, fatty-acid synthases and non-ribosomal peptide synthases require post-translational modification of their constituent acyl-carrier-protein (ACP) domains to become catalytically active.
  • The inactive apo-proteins are converted into their active holo-forms by transfer of the 4'-phosphopantetheinyl moiety of CoA to the sidechain hydroxy group of a conserved serine residue in each ACP domain.
  • The enzyme from human can activate both the ACP domain of the human cytosolic multifunctional fatty acid synthase and that associated with human mitochondria as well as peptidyl-carrier and acyl-carrier- proteins from prokaryotes.
  • Removal of the 4-phosphopantetheinyl moiety from holo-ACP is carried out by EC 3.1.4.14.
Systematic name:
CoA-(4'-phosphopantetheine):apo-[acyl-carrier-protein] 4'-pantetheinephosphotransferase
Alternative Name(s):
  • Acyl carrier protein holoprotein (holo-ACP) synthetase
  • Acyl carrier protein synthetase
  • Coenzyme A:fatty acid synthetase apoenzyme 4'-phosphopantetheine transferase
  • Holo-ACP synthase
  • Holo-ACP synthetase
  • Holosynthase
  • PPTase
  • AcpS
  • ACPS
  • Acyl carrier protein synthase
  • P-pant transferase
  • CoA:apo-[acyl-carrier-protein] pantetheinephosphotransferase
  • L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase
  • 4'-phosphopantetheinyl transferase
  • Alpha-aminoadipic semialdehyde dehydrogenase-phosphopantetheinyl transferase

Sequence family

Pfam Protein family (Pfam)
PF01648
Domain description: 4'-phosphopantetheinyl transferase superfamily
Occurring in:
  1. Holo-[acyl-carrier-protein] synthase
1 copy of Pfam domain PF01648 (4'-phosphopantetheinyl transferase superfamily) in Holo-[acyl-carrier-protein] synthase in PDB 1f7l.

InterPro InterPro annotations
IPR002582
Domain description: Holo-[acyl carrier protein] synthase
Occurring in:
  1. Holo-[acyl-carrier-protein] synthase
IPR037143
Domain description: 4'-phosphopantetheinyl transferase domain superfamily
Occurring in:
  1. Holo-[acyl-carrier-protein] synthase
IPR004568
Domain description: Phosphopantetheine-protein transferase domain
Occurring in:
  1. Holo-[acyl-carrier-protein] synthase
IPR008278
Domain description: 4'-phosphopantetheinyl transferase domain
Occurring in:
  1. Holo-[acyl-carrier-protein] synthase

Structure domain

CATH CATH domain
3.90.470.20
Class: Alpha Beta
Architecture: Alpha-Beta Complex
Topology: Ribosomal Protein L22; Chain A
Homology: 4'-phosphopantetheinyl transferase
Occurring in:
  1. Holo-[acyl-carrier-protein] synthase
1 copy of CATH domain 3.90.470.20 (Ribosomal Protein L22; Chain A) in Holo-[acyl-carrier-protein] synthase in PDB 1f7l.
SCOP SCOP annotation
64418
Class: Alpha and beta proteins (a+b)
Fold: 4'-phosphopantetheinyl transferase
Superfamily: 4'-phosphopantetheinyl transferase
Occurring in:
  1. Holo-[acyl-carrier-protein] synthase
1 copy of SCOP domain 64418 (Holo-(acyl carrier protein) synthase ACPS) in Holo-[acyl-carrier-protein] synthase in PDB 1f7l.