PDBe 1f75

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE OF UNDECAPRENYL DIPHOSPHATE SYNTHASE FROM MICROCOCCUS LUTEUS B-P 26

Released:
Source organism: Micrococcus luteus
Primary publication:
Crystal structure of cis-prenyl chain elongating enzyme, undecaprenyl diphosphate synthase.
Proc. Natl. Acad. Sci. U.S.A. 98 4337-42 (2001)
PMID: 11287651

Function and Biology Details

Reaction catalysed:
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate = 8 diphosphate + di-trans,octa-cis-undecaprenyl diphosphate. 
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ditrans,polycis-undecaprenyl-diphosphate synthase ((2E,6E)-farnesyl-diphosphate specific) Chains: A, B
Molecule details ›
Chains: A, B
Length: 249 amino acids
Theoretical weight: 28.95 KDa
Source organism: Micrococcus luteus
Expression system: Escherichia coli
UniProt:
  • Canonical: O82827 (Residues: 1-249; Coverage: 100%)
Gene name: uppS
Sequence domains: Putative undecaprenyl diphosphate synthase
Structure domains: Undecaprenyl pyrophosphate synthetase

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6B
Spacegroup: C2
Unit cell:
a: 127.197Å b: 60.161Å c: 75.703Å
α: 90° β: 105.69° γ: 90°
R-values:
R R work R free
0.19 0.19 0.246
Expression system: Escherichia coli