› 1f4u

X-ray diffraction
2.69Å resolution

THERMOPHILIC P450: CYP119 FROM SULFOLOBUS SOLFACTARICUS

Released:
DOI: 10.2210/pdb1f4u/pdb
PDB entry 1f4u coloured by chain, front view.
PDB entry 1f4u coloured by chain, side view.
PDB entry 1f4u coloured by chain, top view.
Source organism: Saccharolobus solfataricus
Primary publication:
Crystal structure of a thermophilic cytochrome P450 from the archaeon Sulfolobus solfataricus.
Yano JK, Koo LS, Schuller DJ, Li H, Ortiz de Montellano PR, Poulos TL
J Biol Chem 275 31086-92 (2000)
PMID: 10859321

Function and Biology Details

Reaction catalysed: 
2 phenolic donor + H(2)O(2) = 2 phenoxyl radical of the donor + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176263 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P450 119 Chains: A, B
Molecule details ›
Chains: A, B
Length: 368 amino acids
Theoretical weight: 42.92 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q55080 (Residues: 1-368; Coverage: 100%)
Gene names: Saci_2081, cyp119
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments


Cofactor: Ligand HEM 2 x HEM
2 bound ligands:
Ligand SO4 2 x SO4
Ligand IMD 2 x IMD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P21
Unit cell:
a: 76.969Å b: 70.258Å c: 107.538Å
α: 90° β: 102.35° γ: 90°
R-values:
R R work R free
0.219 0.219 0.253
Expression system: Escherichia coli

Quick links

Citations

21 review citations

Hydrocarbon hydroxylation by cytochrome P450 enzymes.
Ortiz de Montellano PR. (2010)
20 more

7 mentions without citation

Functional and protective hole hopping in metalloenzymes.
Gray et al. (2021)
6 more