PDBe 1f1w

X-ray diffraction
2.1Å resolution

SRC SH2 THREF1TRP MUTANT COMPLEXED WITH THE PHOSPHOPEPTIDE S(PTR)VNVQN

Released:

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. 
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
S(PTR)VNVQN PHOSPHOPEPTIDE Chain: B
Molecule details ›
Chain: B
Length: 7 amino acids
Theoretical weight: 903 Da
Source organism: Synthetic construct
Expression system: Not provided
UniProt:
Proto-oncogene tyrosine-protein kinase Src Chain: A
Molecule details ›
Chain: A
Length: 104 amino acids
Theoretical weight: 12.06 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00523 (Residues: 144-247; Coverage: 20%)
Gene name: SRC
Sequence domains: SH2 domain
Structure domains: SHC Adaptor Protein

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-C
Spacegroup: C2221
Unit cell:
a: 33.714Å b: 56.804Å c: 102.779Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.195 0.257
Expression systems:
  • Not provided
  • Escherichia coli