PDBe 1ezv

X-ray diffraction
2.3Å resolution

STRUCTURE OF THE YEAST CYTOCHROME BC1 COMPLEX CO-CRYSTALLIZED WITH AN ANTIBODY FV-FRAGMENT

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 20-mer (preferred)
Entry contents:
11 distinct polypeptide molecules
Macromolecules (11 distinct):
Cytochrome b Chain: C
Molecule details ›
Chain: C
Length: 385 amino acids
Theoretical weight: 43.67 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
UniProt:
  • Canonical: P00163 (Residues: 1-385; Coverage: 100%)
Gene names: COB, CYTB, Q0105
Sequence domains:
Structure domains: Cytochrome Bc1 Complex; Chain C
Cytochrome c1, heme protein, mitochondrial Chain: D
Molecule details ›
Chain: D
Length: 245 amino acids
Theoretical weight: 27.42 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
UniProt:
  • Canonical: P07143 (Residues: 62-306; Coverage: 79%)
Gene names: CTC1, CYT1, YOR065W, YOR29-16
Sequence domains: Cytochrome C1 family
Structure domains:
Ig heavy chain V region 3-6 Chain: X
Molecule details ›
Chain: X
Length: 127 amino acids
Theoretical weight: 14.37 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P18531 (Residues: 19-116; Coverage: 100%)
Gene names: Gm16932, Ighv3-6
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
Ig kappa chain V-V region HP 124E1 Chain: Y
Molecule details ›
Chain: Y
Length: 107 amino acids
Theoretical weight: 11.93 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01647 (Residues: 1-107; Coverage: 99%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
Cytochrome b-c1 complex subunit Rieske, mitochondrial Chain: E
Molecule details ›
Chain: E
Length: 185 amino acids
Theoretical weight: 20.12 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
UniProt:
  • Canonical: P08067 (Residues: 31-215; Coverage: 86%)
Gene names: RIP1, YEL024W
Sequence domains: Rieske [2Fe-2S] domain
Structure domains:
Cytochrome b-c1 complex subunit 7 Chain: F
Molecule details ›
Chain: F
Length: 125 amino acids
Theoretical weight: 14.36 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
UniProt:
  • Canonical: P00128 (Residues: 3-127; Coverage: 98%)
Gene names: CRO1, D9719.32, QCR7, UCR7, YDR529C
Sequence domains: Ubiquinol-cytochrome C reductase complex 14kD subunit
Structure domains: Cytochrome Bc1 Complex; Chain F
Cytochrome b-c1 complex subunit 6 Chain: H
Molecule details ›
Chain: H
Length: 74 amino acids
Theoretical weight: 8.85 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
UniProt:
  • Canonical: P00127 (Residues: 74-147; Coverage: 50%)
Gene names: QCR6, UCR6, YFR033C
Sequence domains: Ubiquinol-cytochrome C reductase hinge protein
Structure domains: Helix Hairpins
Cytochrome b-c1 complex subunit 9 Chain: I
Molecule details ›
Chain: I
Length: 55 amino acids
Theoretical weight: 6.3 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
UniProt:
  • Canonical: P22289 (Residues: 4-58; Coverage: 83%)
Gene names: QCR9, UCR9, YGR183C
Sequence domains: Ubiquinol-cytochrome C reductase, UQCRX/QCR9 like
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Cytochrome b-c1 complex subunit 2, mitochondrial Chain: B
Molecule details ›
Chain: B
Length: 352 amino acids
Theoretical weight: 38.75 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
UniProt:
  • Canonical: P07257 (Residues: 17-368; Coverage: 96%)
Gene names: COR2, P9677.6, QCR2, UCR2, YPR191W
Sequence domains: Insulinase (Peptidase family M16)
Structure domains: Cytochrome Bc1 Complex; Chain A, domain 1
Cytochrome b-c1 complex subunit 1, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 430 amino acids
Theoretical weight: 47.36 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
UniProt:
  • Canonical: P07256 (Residues: 27-457; Coverage: 94%)
Gene names: COR1, QCR1, YBL0403, YBL045C
Sequence domains: Peptidase M16 inactive domain
Structure domains: Cytochrome Bc1 Complex; Chain A, domain 1
Cytochrome b-c1 complex subunit 8 Chain: G
Molecule details ›
Chain: G
Length: 93 amino acids
Theoretical weight: 10.86 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
UniProt:
  • Canonical: P08525 (Residues: 2-94; Coverage: 99%)
Gene names: J0526, QCR8, YJL166W
Sequence domains: UcrQ family
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: C2
Unit cell:
a: 214.47Å b: 163.92Å c: 147.27Å
α: 90° β: 117.5° γ: 90°
R-values:
R R work R free
0.223 0.222 0.254
Expression systems:
  • Not provided
  • Escherichia coli