PDBe 1ezf

X-ray diffraction
2.15Å resolution

CRYSTAL STRUCTURE OF HUMAN SQUALENE SYNTHASE

Released:

Function and Biology Details

Reaction catalysed:
2 farnesyl diphosphate + NAD(P)H = squalene + 2 diphosphate + NAD(P)(+). 
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Squalene synthase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 340 amino acids
Theoretical weight: 39.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P37268 (Residues: 31-370; Coverage: 82%)
  • Best match: P37268-2 (Residues: 1-306)
Gene name: FDFT1
Sequence domains: Squalene/phytoene synthase
Structure domains: Farnesyl Diphosphate Synthase

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 76.76Å b: 87.92Å c: 154.17Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.213 0.262
Expression system: Escherichia coli