PDBe 1esk

Solution NMR

SOLUTION STRUCTURE OF NCP7 FROM HIV-1

Released:
Source organism: Synthetic construct
Entry authors: Morellet N, Demene H, Teilleux V, Huynh-Dinh T, de Rocquigny H, Fournie-Zaluski M-C, Roques BP

Function and Biology Details

Reactions catalysed:
Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus. 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). 
Specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro. 
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid. 
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nucleocapsid protein p7 Chain: A
Molecule details ›
Chain: A
Length: 42 amino acids
Theoretical weight: 4.84 KDa
Source organism: Synthetic construct
Expression system: Not provided
UniProt:
  • Canonical: P04585 (Residues: 389-430; Coverage: 3%)
Gene name: gag-pol
Sequence domains: Zinc knuckle
Structure domains: HIV-1 Nucleocapsid Protein

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Not provided