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Structure of the epididymal retinoic acid binding protein at 2.1 A resolution.

Newcomer ME
Structure 1 7-18 (1993)
Cited: 55 times
EuropePMC logo PMID: 8069623

Abstract

Background

Androgen-dependent proteins in the lumen of the epididymis are required for sperm maturation. One of these is a retinoic acid binding protein, E-RABP, which binds both all-trans and 9-cis retinoic acid. The other retinoid-binding proteins whose structures are known do not bind 9-cis retinoids.

Results

We describe the X-ray structure determination of E-RABP with and without bound ligand. The ligand binds deep in the beta-barrel of the protein, the beta-ionone ring innermost. The binding site, like the ligand, is amphipathic and the deepest part of the cavity is formed by a ring of aromatic amino acids. The isoprene tail of all-trans retinoic acid is bound in a folded conformation which resembles that of the 9-cis isomer.

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Reviews citing this publication (11)

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Related citations provided by authors (1)

  1. X-Ray Crystallographic Identification of a Protein Binding Site for Both All-Trans-and 9-Cis-Retinoic Acid. Newcomer ME, Pappas RS, Ong DE Proc. Natl. Acad. Sci. U.S.A. 90 9223- (1993)

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