PDBe 1eom

X-ray diffraction
2.1Å resolution

CRYSTAL STRUCTURE OF THE COMPLEX OF ENDO-BETA-N-ACETYLGLUCOSAMINIDASE F3 WITH A BIANTENNARY COMPLEX OCTASACCHARIDE

Released:

Function and Biology Details

Reaction catalysed:
Endohydrolysis of the N,N'-diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -(Man(GlcNAc)(2))Asn- structure. One N-acetyl-D-glucosamine residue remains attached to the protein; the rest of the oligosaccharide is released intact. 
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endo-beta-N-acetylglucosaminidase F3 Chain: A
Molecule details ›
Chain: A
Length: 290 amino acids
Theoretical weight: 31.56 KDa
Source organism: Elizabethkingia meningoseptica
Expression system: Escherichia coli
UniProt:
  • Canonical: P36913 (Residues: 40-329; Coverage: 100%)
Gene name: endOF3
Sequence domains: Glycosyl hydrolases family 18
Structure domains: Glycosidases

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 68.994Å b: 117.456Å c: 40.156Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.161 0.215
Expression system: Escherichia coli