Function and Biology

ENDONUCLEASE VII (ENDOVII) FROM PHAGE T4

Source organism: Enterobacteria phage T4
Biochemical function: metal ion binding
Biological process: nucleic acid phosphodiester bond hydrolysis
Cellular component: not assigned

EC 3.1.22.4: Crossover junction endodeoxyribonuclease

Reaction catalysed:
Endonucleolytic cleavage at a junction such as a reciprocal single- stranded crossover between two homologous DNA duplexes (Holliday junction).
Comments:
  • The enzyme from Saccharomyces cerevisiae has no endonuclease or exonuclease activity on single-stranded or double-stranded DNA molecules that do not contain Holliday junctions.
Alternative Name(s):
  • Hje endonuclease
  • Holliday junction endonuclease CCE1
  • Holliday junction resolvase
  • Holliday junction-cleaving endonuclease
  • Holliday junction-resolving endoribonuclease
  • RusA Holliday junction resolvase
  • RusA endonuclease
  • RuvC endonuclease
  • SpCCe1 Holliday junction resolvase
  • Crossover junction endoribonuclease
  • Cruciform-cutting endonuclease
  • Endo X3
  • Endonuclease RuvC
  • Endonuclease VII
  • Endonuclease X3
  • Resolving enzyme CCE1
  • Holliday junction nuclease

GO terms

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Sequence families

CATH Protein families (Pfam)
PF09124
Domain description: T4 recombination endonuclease VII, dimerisation
Occurring in:
  1. Recombination endonuclease VII
1 copy of Pfam domain PF09124 (T4 recombination endonuclease VII, dimerisation) in Recombination endonuclease VII in PDB 1en7.

PF02945
Domain description: Recombination endonuclease VII
Occurring in:
  1. Recombination endonuclease VII
1 copy of Pfam domain PF02945 (Recombination endonuclease VII) in Recombination endonuclease VII in PDB 1en7.

CATH InterPro annotations
IPR004211
Domain description: Recombination endonuclease VII
Occurring in:
  1. Recombination endonuclease VII
IPR015208
Domain description: T4 recombination endonuclease VII, dimerisation
Occurring in:
  1. Recombination endonuclease VII

Structure domains

CATH CATH domains
1.10.720.10
Class: Mainly Alpha
Architecture: Orthogonal Bundle
Topology: Transcription Termination Factor Rho, Rna-binding Domain; Chain A, Domain 1
Homology: Transcription Termination Factor Rho, Rna-binding Domain; Chain A, Domain 1
Occurring in:
  1. Recombination endonuclease VII
1 copy of CATH domain 1.10.720.10 (Transcription Termination Factor Rho, Rna-binding Domain; Chain A, Domain 1) in Recombination endonuclease VII in PDB 1en7.
3.40.1800.10
Class: Alpha Beta
Architecture: 3-Layer(aba) Sandwich
Topology: His-Me finger endonuclease fold
Homology: His-Me finger endonucleases
Occurring in:
  1. Recombination endonuclease VII
1 copy of CATH domain 3.40.1800.10 (His-Me finger endonuclease fold) in Recombination endonuclease VII in PDB 1en7.
SCOP SCOP annotations
68919
Class: All alpha proteins
Fold: LEM/SAP HeH motif
Superfamily: Recombination endonuclease VII, C-terminal and dimerization domains
Occurring in:
  1. Recombination endonuclease VII
1 copy of SCOP domain 68919 (Recombination endonuclease VII, C-terminal and dimerization domains) in Recombination endonuclease VII in PDB 1en7.
68915
Class: Alpha and beta proteins (a+b)
Fold: His-Me finger endonucleases
Superfamily: His-Me finger endonucleases
Occurring in:
  1. Recombination endonuclease VII
1 copy of SCOP domain 68915 (Recombination endonuclease VII, N-terminal domain) in Recombination endonuclease VII in PDB 1en7.