PDBe 1en7

X-ray diffraction
2.4Å resolution

ENDONUCLEASE VII (ENDOVII) FROM PHAGE T4

Released:

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage at a junction such as a reciprocal single- stranded crossover between two homologous DNA duplexes (Holliday junction). 
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Recombination endonuclease VII Chains: A, B
Molecule details ›
Chains: A, B
Length: 157 amino acids
Theoretical weight: 18.17 KDa
Source organism: Enterobacteria phage T4
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P13340 (Residues: 1-157; Coverage: 100%)
Gene name: 49
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS
Spacegroup: C2
Unit cell:
a: 144.99Å b: 39.45Å c: 75.75Å
α: 90° β: 106.2° γ: 90°
R-values:
R R work R free
0.24 0.24 0.308
Expression system: Escherichia coli BL21