PDBe 1ejx

X-ray diffraction
1.6Å resolution

CRYSTAL STRUCTURE OF WILD-TYPE KLEBSIELLA AEROGENES UREASE AT 100K

Released:
Source organism: Klebsiella aerogenes
Entry authors: Pearson MA, Karplus PA

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Urease subunit beta Chain: B
Molecule details ›
Chain: B
Length: 101 amino acids
Theoretical weight: 11.13 KDa
Source organism: Klebsiella aerogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: P18315 (Residues: 1-101; Coverage: 95%)
Gene name: ureB
Sequence domains: Urease beta subunit
Structure domains: Urease, beta-subunit
Urease subunit alpha Chain: C
Molecule details ›
Chain: C
Length: 567 amino acids
Theoretical weight: 60.41 KDa
Source organism: Klebsiella aerogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: P18314 (Residues: 1-567; Coverage: 100%)
Gene name: ureC
Sequence domains:
Structure domains:
Urease subunit gamma Chain: A
Molecule details ›
Chain: A
Length: 100 amino acids
Theoretical weight: 11.1 KDa
Source organism: Klebsiella aerogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: P18316 (Residues: 1-100; Coverage: 100%)
Gene name: ureA
Sequence domains: Urease, gamma subunit
Structure domains: Urease, gamma-subunit

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: I213
Unit cell:
a: 168.96Å b: 168.96Å c: 168.96Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.172 0.24
Expression system: Escherichia coli