PDBe 1ehy

X-ray diffraction
2.1Å resolution

X-ray structure of the epoxide hydrolase from agrobacterium radiobacter ad1

Released:

Function and Biology Details

Reaction catalysed:
An epoxide + H(2)O = a glycol. 
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Epoxide hydrolase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 294 amino acids
Theoretical weight: 34.07 KDa
Source organism: Agrobacterium tumefaciens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O31243 (Residues: 1-1, 3-294; Coverage: 100%)
Gene name: echA
Sequence domains:
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: C2
Unit cell:
a: 146.624Å b: 100.201Å c: 96.879Å
α: 90° β: 100.68° γ: 90°
R-values:
R R work R free
0.19 0.19 0.227
Expression system: Escherichia coli BL21(DE3)