PDBe 1eh6

X-ray diffraction
2Å resolution

HUMAN O6-ALKYLGUANINE-DNA ALKYLTRANSFERASE

Released:

Function and Biology Details

Reaction catalysed:
(1) DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine. (2) DNA (containing 4-O-methylthymine) + protein L-cysteine = DNA (without 4-O-methylthymine) + protein S-methyl-L-cysteine. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methylated-DNA--protein-cysteine methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 207 amino acids
Theoretical weight: 21.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P16455 (Residues: 1-207; Coverage: 100%)
Gene name: MGMT
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P3121
Unit cell:
a: 71.283Å b: 71.283Å c: 73.611Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.197 0.197 0.218
Expression system: Escherichia coli