PDBe 1efn

X-ray diffraction
2.5Å resolution

HIV-1 NEF PROTEIN IN COMPLEX WITH R96I MUTANT FYN SH3 DOMAIN

Released:

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. 
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
C-terminal core protein Chains: B, D
Molecule details ›
Chains: B, D
Length: 152 amino acids
Theoretical weight: 17.81 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Escherichia coli
UniProt:
  • Canonical: P03406 (Residues: 54-205; Coverage: 74%)
Gene name: nef
Structure domains: Nef Regulatory Factor
Tyrosine-protein kinase Fyn Chains: A, C
Molecule details ›
Chains: A, C
Length: 59 amino acids
Theoretical weight: 6.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06241 (Residues: 85-143; Coverage: 11%)
Gene name: FYN
Sequence domains: SH3 domain
Structure domains: SH3 Domains

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P6522
Unit cell:
a: 107.8Å b: 107.8Å c: 229.1Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.215 0.215 0.282
Expression systems:
  • Escherichia coli
  • Escherichia coli BL21(DE3)