› 1ed8

X-ray diffraction
1.75Å resolution

STRUCTURE OF E. COLI ALKALINE PHOSPHATASE INHIBITED BY THE INORGANIC PHOSPHATE AT 1.75A RESOLUTION

Released:
DOI: 10.2210/pdb1ed8/pdb
PDB entry 1ed8 coloured by chain, front view.
PDB entry 1ed8 coloured by chain, side view.
PDB entry 1ed8 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
A revised mechanism for the alkaline phosphatase reaction involving three metal ions.
Stec B, Holtz KM, Kantrowitz ER
J Mol Biol 299 1303-11 (2000)
PMID: 10873454

Function and Biology Details

Reaction catalysed: 
A phosphate monoester + H(2)O = an alcohol + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132772 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alkaline phosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 449 amino acids
Theoretical weight: 47.09 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P00634 (Residues: 23-471; Coverage: 100%)
Gene names: JW0374, b0383, phoA
Sequence domains: Alkaline phosphatase
Structure domains: Alkaline Phosphatase, subunit A

Ligands and Environments

4 bound ligands:
Ligand ZN 6 x ZN
Ligand MG 2 x MG
Ligand PO4 2 x PO4
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: I222
Unit cell:
a: 195.15Å b: 167.34Å c: 76.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.196 0.228
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Cellular function and molecular structure of ecto-nucleotidases.
Zimmermann et al. (2012)
14 more

13 mentions without citation

Prediction of Protein Loop Conformations using the AGBNP Implicit Solvent Model and Torsion Angle Sampling.
Felts et al. (2008)
12 more