PDBe 1ecl

X-ray diffraction
1.9Å resolution

AMINO TERMINAL 67KDA DOMAIN OF ESCHERICHIA COLI DNA TOPOISOMERASE I (RESIDUES 2-590 OF MATURE PROTEIN) CLONING ARTIFACT ADDS TWO RESIDUES TO THE AMINO-TERMINUS WHICH WERE NOT OBSERVED IN THE EXPERIMENTAL ELECTRON DENSITY (GLY-2, SER-1).

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA topoisomerase 1 Chain: A
Molecule details ›
Chain: A
Length: 597 amino acids
Theoretical weight: 67.33 KDa
Source organism: Escherichia coli
Expression system: Not provided
UniProt:
  • Canonical: P06612 (Residues: 1-597; Coverage: 69%)
Gene names: JW1266, b1274, supX, topA
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CORNELL HIGH ENERGY SYNCHROTRON SOURCE
Spacegroup: P212121
Unit cell:
a: 63.19Å b: 77.97Å c: 138.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.218 not available
Expression system: Not provided