PDBe 1e7l

X-ray diffraction
1.32Å resolution

Endonuclease VII (EndoVII) N62D mutant from phage T4

Released:

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage at a junction such as a reciprocal single- stranded crossover between two homologous DNA duplexes (Holliday junction). 
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Recombination endonuclease VII Chains: A, B
Molecule details ›
Chains: A, B
Length: 157 amino acids
Theoretical weight: 18.17 KDa
Source organism: Enterobacteria phage T4
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P13340 (Residues: 1-157; Coverage: 100%)
Gene name: 49
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P21
Unit cell:
a: 58.24Å b: 35.83Å c: 91.93Å
α: 90° β: 103.93° γ: 90°
R-values:
R R work R free
0.145 0.146 0.186
Expression system: Escherichia coli BL21