› 1e6z

X-ray diffraction
1.99Å resolution

CHITINASE B FROM SERRATIA MARCESCENS WILDTYPE IN COMPLEX WITH CATALYTIC INTERMEDIATE

Released:
DOI: 10.2210/pdb1e6z/pdb
PDB entry 1e6z coloured by chain, front view.
PDB entry 1e6z coloured by chain, side view.
PDB entry 1e6z coloured by chain, top view.
Source organism: Serratia marcescens
Primary publication:
Structural insights into the catalytic mechanism of a family 18 exo-chitinase.
van Aalten DM, Komander D, Synstad B, Gåseidnes S, Peter MG, Eijsink VG
Proc Natl Acad Sci U S A 98 8979-84 (2001)
PMID: 11481469

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176194 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
GH18 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 498 amino acids
Theoretical weight: 55.39 KDa
Source organism: Serratia marcescens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q54276 (Residues: 2-499; Coverage: 100%)
Gene name: chiB
Sequence domains: Glycosyl hydrolases family 18
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
3 bound ligands:
Ligand SO4 4 x SO4
Ligand NGO 2 x NGO
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: P212121
Unit cell:
a: 55.801Å b: 103.811Å c: 186.359Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.187 0.225
Expression system: Escherichia coli

Quick links

Citations

22 review citations

Lysozymes in the animal kingdom.
Callewaert et al. (2010)
21 more

2 mentions without citation

Structural insights into the catalytic mechanism of a family 18 exo-chitinase.
van Aalten et al. (2001)
1 more