PDBe 1e6e

X-ray diffraction
2.3Å resolution

ADRENODOXIN REDUCTASE/ADRENODOXIN COMPLEX OF MITOCHONDRIAL P450 SYSTEMS

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Adrenodoxin, mitochondrial Chains: B, D
Molecule details ›
Chains: B, D
Length: 128 amino acids
Theoretical weight: 14.03 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P00257 (Residues: 59-186; Coverage: 69%)
  • Best match: P00257-2 (Residues: 61-186)
Gene names: ADX, FDX1
Sequence domains: 2Fe-2S iron-sulfur cluster binding domain
Structure domains: Ubiquitin-like (UB roll)
NADPH:adrenodoxin oxidoreductase, mitochondrial Chains: A, C
Molecule details ›
Chains: A, C
Length: 460 amino acids
Theoretical weight: 50.36 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P08165 (Residues: 33-492; Coverage: 94%)
Gene names: ADXR, FDXR
Sequence domains: Pyridine nucleotide-disulphide oxidoreductase
Structure domains:

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P6522
Unit cell:
a: 92.21Å b: 92.21Å c: 607.85Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.222 0.222 0.268
Expression system: Escherichia coli BL21