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Crystal structure of the inactive mutant Monocot (Maize ZMGlu1) beta-glucosidase ZM Glu191Asp

Source organism: Zea mays
Biochemical function: DIMBOA glucoside beta-D-glucosidase activity
Biological process: cytokinin-activated signaling pathway
Cellular component: plastid

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EC 3.2.1.182: 4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase

Reaction catalysed:
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H(2)O = 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one + D-glucose
Systematic name:
(2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside beta-D-glucosidase
Alternative Name(s):
  • DIMBOA glucosidase
  • DIMBOAGlc hydrolase

EC 3.2.1.21: Beta-glucosidase

Reaction catalysed:
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose
Systematic name:
Beta-D-glucoside glucohydrolase
Alternative Name(s):
  • Amygdalase
  • Amygdalinase
  • Arbutinase
  • Aryl-beta-glucosidase
  • Beta-1,6-glucosidase
  • Beta-D-glucosidase
  • Beta-glucoside glucohydrolase
  • Cellobiase
  • Elaterase
  • Emulsin
  • Gentiobiase
  • Gentobiase
  • Limarase
  • Primeverosidase
  • Salicilinase
  • p-nitrophenyl beta-glucosidase

GO terms

Sequence family

Pfam Protein family (Pfam)
PF00232
Domain description: Glycosyl hydrolase family 1
Occurring in:
  1. 4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase 1, chloroplastic
The deposited structure of PDB entry 1e4l contains 2 copies of Pfam domain PF00232 (Glycosyl hydrolase family 1) in 4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase 1, chloroplastic. Showing 1 copy in chain A.

InterPro InterPro annotations
IPR001360
Domain description: Glycoside hydrolase family 1
Occurring in:
  1. 4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase 1, chloroplastic
IPR018120
Domain description: Glycoside hydrolase family 1, active site
Occurring in:
  1. 4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase 1, chloroplastic
IPR033132
Domain description: Glycosyl hydrolases family 1, N-terminal conserved site
Occurring in:
  1. 4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase 1, chloroplastic
IPR017853
Domain description: Glycoside hydrolase superfamily
Occurring in:
  1. 4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase 1, chloroplastic

Structure domain

CATH CATH domain
3.20.20.80
Class: Alpha Beta
Architecture: Alpha-Beta Barrel
Topology: TIM Barrel
Homology: Glycosidases
Occurring in:
  1. 4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase 1, chloroplastic
The deposited structure of PDB entry 1e4l contains 2 copies of CATH domain 3.20.20.80 (TIM Barrel) in 4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase 1, chloroplastic. Showing 1 copy in chain A.
SCOP SCOP annotation
51521
Class: Alpha and beta proteins (a/b)
Fold: TIM beta/alpha-barrel
Superfamily: (Trans)glycosidases
Occurring in:
  1. 4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase 1, chloroplastic
The deposited structure of PDB entry 1e4l contains 2 copies of SCOP domain 51521 (Family 1 of glycosyl hydrolase) in 4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase 1, chloroplastic. Showing 1 copy in chain A.

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