PDBe 1e3v

X-ray diffraction
2Å resolution

Crystal structure of ketosteroid isomerase from Psedomonas putida complexed with deoxycholate

Released:

Function and Biology Details

Reaction catalysed:
A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Steroid Delta-isomerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 131 amino acids
Theoretical weight: 14.55 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
  • Canonical: P07445 (Residues: 1-131; Coverage: 100%)
Gene name: ksi
Sequence domains: SnoaL-like domain
Structure domains: Nuclear Transport Factor 2; Chain: A,

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: M18XHF
Spacegroup: P212121
Unit cell:
a: 36.58Å b: 75.21Å c: 96.13Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.198 0.264
Expression system: Escherichia coli