PDBe 1e3o

X-ray diffraction
1.9Å resolution

Crystal structure of Oct-1 POU dimer bound to MORE

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
POU domain, class 2, transcription factor 1 Chain: C
Molecule details ›
Chain: C
Length: 160 amino acids
Theoretical weight: 18.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P14859 (Residues: 280-355, 357-438; Coverage: 21%)
Gene names: OCT1, OTF1, POU2F1
Sequence domains:
Structure domains:
5'-D(*AP*TP*GP*CP*AP*TP*GP*AP*GP*GP*A)-3' Chain: A
Molecule details ›
Chain: A
Length: 11 nucleotides
Theoretical weight: 3.42 KDa
5'-D(*TP*CP*CP*TP*CP*AP*TP*GP*CP*AP*T)-3' Chain: B
Molecule details ›
Chain: B
Length: 11 nucleotides
Theoretical weight: 3.28 KDa

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: C2
Unit cell:
a: 93.3Å b: 52.4Å c: 69Å
α: 90° β: 127.6° γ: 90°
R-values:
R R work R free
0.22 0.22 0.242
Expression system: Escherichia coli BL21(DE3)