PDBe 1e2a

X-ray diffraction
2.3Å resolution

ENZYME IIA FROM THE LACTOSE SPECIFIC PTS FROM LACTOCOCCUS LACTIS

Released:

Function and Biology Details

Reaction catalysed:
[Protein]-N(pi)-phospho-L-histidine + lactose(Side 1) = [protein]-L- histidine + lactose 6'-phosphate(Side 2). 
Biochemical function:
Cellular component:

Structure analysis Details

Assemblies composition:
homo trimer (preferred)
homo hexamer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PTS system lactose-specific EIIA component Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 105 amino acids
Theoretical weight: 11.46 KDa
Source organism: Lactococcus lactis
Expression system: Escherichia coli
UniProt:
  • Canonical: P23532 (Residues: 1-105; Coverage: 100%)
Gene name: lacF
Sequence domains: PTS system, Lactose/Cellobiose specific IIA subunit
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X31
Spacegroup: P41212
Unit cell:
a: 90.9Å b: 90.9Å c: 82.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.19 0.24
Expression system: Escherichia coli