› 1e0s

X-ray diffraction
2.28Å resolution

small G protein Arf6-GDP

Released:
DOI: 10.2210/pdb1e0s/pdb
PDB entry 1e0s coloured by chain, front view.
PDB entry 1e0s coloured by chain, side view.
PDB entry 1e0s coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of Arf6-GDP suggests a basis for guanine nucleotide exchange factors specificity.
Ménétrey J, Macia E, Pasqualato S, Franco M, Cherfils J
Nat Struct Biol 7 466-9 (2000)
PMID: 10881192

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158604 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ADP-ribosylation factor 6 Chain: A
Molecule details ›
Chain: A
Length: 174 amino acids
Theoretical weight: 19.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62330 (Residues: 2-175; Coverage: 99%)
Gene name: ARF6
Sequence domains: ADP-ribosylation factor family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand GDP 1 x GDP
Ligand BME 1 x BME
Ligand NH4 1 x NH4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LURE BEAMLINE DW32
Spacegroup: P6122
Unit cell:
a: 55.66Å b: 55.66Å c: 194.95Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.172 0.172 0.234
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Multiple roles for Arf6: sorting, structuring, and signaling at the plasma membrane.
Donaldson JG. (2003)
6 more

23 mentions without citation

Allostery: absence of a change in shape does not imply that allostery is not at play.
Tsai et al. (2008)
22 more